Reversed-phase HPLC methods development can take anywhere from weeks to months, incurring large operational cost. By utilizing UltraPerformance LC (UPLC) Technology for methods development, a 6-fold improvement in throughput can be realized. This, in turn, reduces cost per sample and time of analysis considerably while maintaining or improving separation integrity. By developing rapid, high resolution analytical methods, products can be brought to market faster, therefore, improving the overall profitability of the assay. Here we demonstrate this methods development approach in the separation of paroxetine hydrochloride and its related compounds.