Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology

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Kathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. Rainville
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The demand for highly selective and sensitive LC-MS bioanalytical assays in support of research and development of next-generation oligonucleotide therapies has greatly increased. The work described herein uses SPE, RP-UPLC with the sub-2-μm ACQUITY PREMIER Oligonucleotide C18 Column, and tandem-quadrupole MS for detection and quantification of oligodeoxythymidines and the fully phosphorothioated oligonucleotide antisense therapy, GEM91.

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