A Rapid Method for the Ultra-Sensitive Quantification of Fluticasone Propionate and Salmeterol Xinafoate from Human Plasma

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Nikunj Tanna, Lauren Mullin, and Robert Plumb
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Application Notes
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The method described employs a simple pretreatment and SPE strategy combined with analytical flow LC and tandem-quadrupole MS for the pg/mL level quantification of fluticasone propionate and salmeterol xinafoate from human plasma. The main features of the method include:

  • Simple, fast, and inexpensive sample preparation with Oasis PRiME HLB SPE
  • Use of a sub-2-μm BEH C18 Column for excellent peak shapes and peak width

The analytical sensitivity (0.1 pg/mL and 0.05 pg/mL), linear dynamic range (0.1–10 and 0.05–5 pg/mL), and excellent reproducibility of the method described reliably measures low levels of fluticasone propionate and salmeterol xinafoate.

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