A Rapid Method for the Ultra-Sensitive Quantification of Fluticasone Propionate and Salmeterol Xinafoate from Human Plasma

Library Number:
APNT135022621
Part Number:
720006592EN
Author(s):
Nikunj Tanna, Lauren Mullin, and Robert Plumb
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 

The method described employs a simple pretreatment and SPE strategy combined with analytical flow LC and tandem-quadrupole MS for the pg/mL level quantification of fluticasone propionate and salmeterol xinafoate from human plasma. The main features of the method include:

  • Simple, fast, and inexpensive sample preparation with Oasis PRiME HLB SPE
  • Use of a sub-2-μm BEH C18 Column for excellent peak shapes and peak width

The analytical sensitivity (0.1 pg/mL and 0.05 pg/mL), linear dynamic range (0.1–10 and 0.05–5 pg/mL), and excellent reproducibility of the method described reliably measures low levels of fluticasone propionate and salmeterol xinafoate.


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