Separation of 16 Cannabinoids in Cannabis Flower and Extracts Using a Reversed Phase Isocratic HPLC Method

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Andrew J Aubin, Catharine Layton, and Shawn Helmueller
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Application Notes
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Application Notes
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Several industry standardization bodies are currently developing harmonized methods for cannabinoid profiling to minimize the inherent variability in sampling, extraction, and instrumental analysis with an aim to improve potency result accuracy. High Performance Liquid Chromatography (HPLC) is the preferred methodology for potency determination (Total THC, Total CBD, or THC/CBD ratio) as it can identify and measure the structurally similar cannabinoids and their different forms (e.g. free form and corresponding acid forms of THC and CBD) in a single analysis.

This application note presents a simple isocratic separation of 16 cannabinoids that can be used for the analysis of both plant material and concentrates to ensure the quality and safety of cannabis products.

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