MetaboQuan-R for Bile Acids in Human Serum: A Rapid, Targeted UPLC-MS/MS Method for Metabolomic Research Studies

Library Number:
APNT134977277
Part Number:
720006261EN
Author(s):
Billy Joe Molloy
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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Targeted Metabolomics

This application note demonstrates how these compounds can be separated, without compromising throughput, using Waters® CORTECS UPLC Technology combined with negative mode electrospray ionization mass spectrometry.

A rapid UPLC-MS/MS methodology has been developed for the analysis of bile acids. This research method has been demonstrated to be suitable for the analysis of physiologically relevant levels of these analytes in human serum. This method utilizes a generic LC-MS platform that can be used for various compound classes (including metabolomics, lipidomics, and proteomics), meaning it can be applied as part of a suite of analyses that are run subsequently as part of a targeted multi-omics workflow.


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