Selecting a Reversed-Phase Column for the Peptide Mapping Analysis of a Biotherapeutic Protein

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Stephan M. Koza and Erin E. Chambers
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Application Notes
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Application Notes
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This application note focuses on reversed-phase (RP) column selection considerations for peptide mapping applied to the routine characterization and analysis of biotherapeutic proteins.

In this study, ten distinctly different RP column types, representing variations in ligand characteristics, base particle composition, and particle size, were evaluated.  Among these, we have identified four Waters RP Particle Technologies with notable applicability to peptide mapping. Unlike other options, these column technologies provide both high peak capacity separations and sizeable differences in retentivity and selectivity, which can in turn be used to expedite the development of RP peptide mapping separations.

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