Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer

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Paula Hong and Patricia R. McConville
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Method transfer or method scaling across different LC instrument platforms can be affected by both dwell volume and extra-column dispersion. Each characteristic will have a different effect on the chromatographic separation.

  • Dwell volume impacts the retention times of a gradient separation, but can also affect selectivity, particularly for early eluting compounds.
  • Extra-column dispersion or volume impacts peak width, resolution, and the overall efficiency of a separation.

In this review, the differences of these attributes across of variety of HPLC, UHPLC, and UPLC instruments will be examined, specifically for their impact on transferring a method. We will review strategies to minimize that impact. These recommendations will follow the USP <621> guidelines.

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