This technology brief demonstrates the Tof MRM acquisition of commercially available peptide mixture MSQC1 using ionKey™/Xevo® G2-XS QTof platform for peptide bioanalysis.
The ionKey/Xevo G2-XS is well suited for the peptide characterization and quantitation with excellent sensitivity and linear response. In this way, one can go from characterization to high performance quantitative method using a single MS platform. The use of a simple turnkey microfluidics technology such as ionKey offers high sensitivity. In summary, the coupling of targeted HRMS (Tof MRM with target enhancement) on the Xevo G2-XS HRMS QTof with ionKey microfluidics, offers a powerful, simple workflow for HRMS peptide bioanalysis.