High throughput analysis at microscale: performance of ionKey/MS with Xevo G2-XS QTof under rapid gradient conditions

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Yun Wang Alelynas, Gregory T. Roman, Jay Jhnson, Catalin Doneanu, Mark D. Wrona
Waters, Journal of Applied Bioanalysis
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Application Notes
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Application Notes
Related Products:
High Sensitivity Bioanalysis and Bioequivalence

In this paper, high throughput analysis with 3-minute, rapid gradient conditions is described. It shows the ionKey/MS System with Xevo G2-XS QTof HRMS is a high performance platform that can be used for highly sensitive detection of small molecules and peptides (and other biomolecules).

Extensive testing of representative small molecules and a peptide shows that the system is well-tolerated and exhibits excellent reproducibility and linear response. The iKey™ HSS T3 Separation Device used is robust, withstanding ~2200 injections of prepared human plasma with excellent peak shape and system pressure profile. A 99% solvent savings was realized when compared with an analytical system using a 2.1 mm column with flow rate ranging from 0.6 mL/min to 1.5 mL/min. These data, coupled with examples from the literature, illustrate that the ionKey/MS System with Xevo G2-XS QTof can be used as a full service platform for high throughput analysis and high sensitivity analysis to support all phases of drug discovery and development.

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