To minimize the consumption of sample and solvents, there is a benefit in developing separation methods on a small scale and transferring them to a larger scale. Taking into account the important parameters and applying appropriate scaling factors, users can successfully scale up from analytical chromatography to larger scale preparative separations. In this application note, the analytical scale separation of chicken egg white is used to demonstrate the calculations and techniques used to successfully transfer from a 4.6 mm I.D. analytical column separation to a 19 mm I.D. preparatory column separation.