Transfer of an Isocratic USP Assay from an Agilent 1100 Series LC System to a ACQUITY UPLC H-Class System: Analysis of Tioconazole and Related Impurities

Library Number:

APNT134830915

Part Number:

720005269EN

Author(s):

Paula Hong and Patricia R. McConville

Source:

Waters

Content Type:

Application Notes

Content Subtype:

Technical Notes

Related Products:

Small Molecule Method Development

ACQUITY UPLC H-Class PLUS System

Pharmaceutical

There are 1100 Reasons to Step Up to Waters

Abstract / Summary

In this technology brief an HPLC USP method transfer from an Agilent 1100 Series LC System to an ACQUITY UPLC® H-Class System was demostrated meeting all USP method criteria.

The USP assay for tioconazole, an isocratic separation, was successfully transferred across the systems from different manufacturers. Both systems met all acceptance and system suitability requirements for the USP monograph assay and organic impurities tests and demonstrated comparable separations. These results illustrate the ability to successfully transfer USP methods from HPLC instrumentation to properly configured ACQUITY UPLC H-Class System.

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