Enantioseparation and Detection of Triazole Fungicides in Wheat Grain and Wheat Straw using the ACQUITY UPC2 System and Xevo TQ-S

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Marian Twohig, Michael O’Leary, and Claude Mallet
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Application Notes
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Application Notes
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The safe use of crop protection products is of paramount importance to the agricultural chemicals manufacturing industry. Extensive studies and trials are carried out in support of product registration. These studies ensure that any risks associated with using the agrochemical product are characterized and properly understood so that it can be safely applied to a crop field.

When a crop protection active ingredient (AI) contains one or more stereogenic centers in its structure, the enantioselective behavior must be studied, since it is known that enantiomers can exhibit different bioactivities. Analytical methods used to evaluate the influence of stereochemistry on the degradation dynamics, environmental fate, and final residue levels help to establish a more accurate risk assessment of crop protection products.

In this application note, ACQUITY UPC2 and tandem quadrupole mass spectrometry are used for the trace-level enantioanalysis of five triazole fungicides. We demonstrate that chiral separations using a 3.0-μm chiral stationary phase (CSP) followed by multiple reaction monitoring (MRM) detection enable concentrations of part-per-trillion (ppt) levels to be reproducibly detected and quantified.

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