Simultaneous in vivo Quantification and Metabolite Identification of Plasma Samples Using High Resolution QTof and Routine MSE Data Analysis

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Mark Wrona, Paul Rainville, Eric Langlois, Nigel Ewing, and Julie Laterreur
Waters, Vertex Biopharmaceuticals
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Application Notes
Content Subtype:
Application Notes
Related Products:
Xevo G2 QTof

This application note summarizes the collaboration between Vertex and Waters to test and demonstrate a quan/qual workflow to determine robustness and reliability of QTof measurements, and compare them against the gold-standard tandem quadrupole workflows.

In this example, propranolol was used as a model compound and a pharmacokinetic (PK) rat study (containing standard curves, PK runs, and QCs) was conducted by Vertex Pharmaceuticals using a Xevo G2 QTof MS System for analysis. The sample set was also run at Vertex using a triple quadrupole platform with a typical MRM-based workflow under the same chromatographic conditions to provide a reference comparison data set.

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