Electron Transfer Dissociation of N-linked Glycopeptides from a Recombinant mAb Using SYNAPT G2-S HDMS

Library Number:
APNT134713626
Part Number:
720004481en
Author(s):
Jonathan P. Williams, Jeffery M. Brown, Stephane Houel, Ying Qing Yu, and Weibin Chen
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
SYNAPT G2-S HDMS
 
 
 
 
 

Electron transfer dissociation (ETD) is a powerful fragmentation technique for determining modification sites of labile post-translational modifications (PTMs), which are often difficult to characterize using collision induced dissociation (CID). This application note offers details on glycopeptide characterization using ETD fragmentation.

ETD fragmentation enhanced by a "super charging" reagent improves the overall glycopeptide fragmentation efficiency. The SYNAPT G2-S HDMS System equipped with an ETD source proves to be an indispensible tool for protein glycosylation analysis.


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