Differentiating Carbohydrate Structural and Positional Isomers by Ion-Mobility Mass Spectrometry

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Weibin Chen, Ying-Qing Yu, Asish Chakraborty, Henry Shion, StJohn Skilton
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In this poster from ASMS 2011, a sensitive method, based on travelling wave ion-mobility mass spectrometry (TWIMS), for the rapid characterization of structural and positional carbohydrate is presented.

Structural and positional isomers are separated by travelling wave ion-mobility and subsequently identified by fragmentation spectra, obtained in the transfer cell of the Synapt G2 mass spectrometer, providing more confident structural information.

The collisional cross section (CCS) values of the separated isomers were automatically calculated after the system was calibrated using a polyalanine calibration standard.


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