Characterizing High-Order Structures of Therapeutic Proteins by Electrospray Ion-Mobility MS
This poster, P-106, was shown at Mass Spec 2010 (Practical Applications of Mass Spectrometry in the Biotechnology Industry), Marina Del Ray, CA, Sept. 8-10, 2010.
- Reproducible safety and efficacy profiles for biotherapeutic proteins require manufacturers to produce products with consistent tertiary structures
- Methods for determining changes in higher order structure are invaluable for the assessment of product quality
- In this work, Waters SYNAPT® G2 HDMS™ is used to characterize biotherapeutic protein tertiary structures and data from orthogonal analytical techniques is applied to corroborate the IMS results
- Cross-sectional areas measured by IMS method are in good agreement with those calculated using the MOBCAL computations