At-Column Dilution Application Notes
At-Column Dilution (ACD) is a technique that you could use in all forms of liquid chromatography, preferably for reversed-phase preparative separations. It was developed specifically for injecting relatively large volumes of relatively strong sample diluents. Such injections may distort the chromatography in a conventional system. If injection artifacts are limiting mass capacity or chromatographic resolution, the effects can be ameliorated by applying At-Column Dilution. In addition, At-Column Dilution often increases system ruggedness and column life by preventing bulk precipitation in the sample loop or in the column itself.