RNAi Duplex Analysis and Purification

Library Number:
APNT10086413
Part Number:
720002800en
Author(s):
Sean M. McCarthy and Martin Gilar
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
ACQUITY SQD
 
 
ACQUITY SQD
 

In this application note, we outline a method utilizing the Waters ACQUITY UPLC System with Oligonucleotide Separation Technology (OST) Columns for the simultaneous annealing and purification of RNAi duplexes in a single step. This method allows for sequential injection of complementary ssRNA molecules, which tightly focus on the column, anneal, and elute as a duplex.


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