Analysis of Antibody Drug Conjugates (ADCs) by Native Mass Spectrometry on the BioAccord System

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Henry Shion, Ying Qing Yu, and Weibin Chen
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Here we demonstrate the performance of the BioAccord™ System for the analysis of antibody drug conjugates (ADCs) under native conditions.

The BioAccord System is a small footprint, high performance bench top system that was designed and developed with simplified user interface as well as automatic system setup and self-diagnostic capabilities.

The obtained result shows that BioAccord System is well suited for routine LC(SEC)-native MS analysis of both cysteine and lysine conjugated ADCs. The average DARs and drug loading distribution results were comparable to that generated from Hydrophobic Interaction Chromatography (HIC) separation (with TUV detection), and results from previous generations of QTof MS systems. Simplified system operation and configurable compliance features will enable the BioAccord System to be readily adopted by scientists with less MS experience, which will allow organizations to more readily deploy the mass spectrometry in supporting ADC across development and manufacturing processes.

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