There is increased concern regarding the authenticity of basmati rice throughout the world. For years, traders have been passing off a lesser quality rice as the world’s finest long-grained, aromatic rice. A DNA rice authenticity verification service in India has concluded that more than 30% of the basmati rice sold in the retail markets of the US and Canada is adulterated with inferior quality grains. In Britain, the Food Standard Agency found in 2005 that about half of all basmati rice sold was adulterated with other strains of long-grain rice. Genuine basmati rice is grown in the foothills of the Himalayas. What external factors play a part in the growth of the rice? Can a basmati strain grown elsewhere in the world can be classed as basmati rice? In this application note, a proof of principle method has been established to assess the authenticity of basmati rice using off the shelf supermarket samples. The combined use of APGC, SYNAPT G2-Si and Progenesis QI Software allow a flexible configuration that can be used for food fraud and food authenticity applications.