Development of a Quantitative SPE-LC/MS/MS Assay for Teriparatide in Human Plasma

Library Number:

APNT134729844

Part Number:

720004588EN

Author(s):

Erin E. Chambers, Mary E. Lame, Jon Bardsley, Sally Hannam, Eileen Collins, Kenneth J. Fountain, Elizabeth Thomas

Source:

Waters, ICON PLC

Content Type:

Application Notes

Content Subtype:

Application Notes

Related Products:

MassLynx MS Software

TargetLynx

ACQUITY UPLC System

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Xevo TQ-S

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CSH (Charged Surface Hybrid) Technology

Abstract / Summary

This study provides a single, simple method for the quantification of teriparatide in human plasma. The method uses UPLC and fast, selective sample prep in 96-well format to achieve a limit of detection (LOD) of 15 pg/mL, a dynamic range of 15 to 500 pg/mL, and an average QC accuracy of 97.5% (across six sources of matrix) from 200 μL of human plasma.

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