Advantages of Online 2D Chromatography for MRM Quantification of Therapeutic Monoclonal Antibodies in Serum

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Catalin Doneanu, Paul Rainville, and Robert S. Plumb
Waters Corporation
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Application Notes
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Application Notes
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Ultra High Sensitivity Bioanalysis

In this application note, we demonstrate that a two-dimensional chromatographic assay (2D LC or 2D UPLC) for mulitple reaction monitoring (MRM) quantification in protein bioanalysis can provide up to a three-fold increase in sensitivity compared to one-dimensional LC/MRM. Additionally, high pH/low pH RP/RP separations can significantly reduce analyte suppression.

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