Advantages of Online 2D Chromatography for MRM Quantification of Therapeutic Monoclonal Antibodies in Serum

Library Number:
APNT134717777
Part Number:
720004510en
Author(s):
Catalin Doneanu, Paul Rainville, and Robert S. Plumb
Source:
Waters Corporation
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
Ultra High Sensitivity Bioanalysis
 
 
 
 

In this application note, we demonstrate that a two-dimensional chromatographic assay (2D LC or 2D UPLC) for mulitple reaction monitoring (MRM) quantification in protein bioanalysis can provide up to a three-fold increase in sensitivity compared to one-dimensional LC/MRM. Additionally, high pH/low pH RP/RP separations can significantly reduce analyte suppression.


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