Symmetry300 Columns

Created around Waters proven silica-based, Symmetry^® reversed-phase HPLC chemistries, these wide-pore, 300Å columns are well suited for the separation, lab-scale isolation, and HPLC analysis of proteins and other biological compounds.

• Ideal for protein/peptide analysis
• Excellent peak shape (UV and MS)
• High peak capacity

Designed to provide maximum reproducibility and material recovery of protein and peptide assays, the columns are available in two particle sizes and chemistries:

Particle Sizes	3.5 µm	5 µm
Chemistries	C4 for large peptides and proteins	C18 for smaller peptides

Pore Size Effects on Resolution – Symmetry 100Å and Symmetry300

Protein: Symmetry300 C₄ Versus Competitors

Symmetry^® Columns are synonymous with reproducible chromatographic results. 
Narrow column specifications help you to achieve reproducible results. The surface coverage requirements of Symmetry Columns are the tightest specifications in the industry, resulting in minimal shifts in resolution and more reproducibility from batch-to-batch and column-to-column.

Unmatched Year-to-Year Reproducibility

SymmetryShield™ columns are reversed-phase columns based on Waters’ patented embedded polar group technology that literally “shields” the silica’s residual silanols from highly basic analytes.
Shield Technology

Improved Peak Shape and Unique Selectivity

 

Symmetry300™ columns are wide-pore, reversed-phase columns built on the Symmetry^® column platform, the standard relied on by the pharmaceutical industry  for sensitive, rugged and robust HPLC analyses.  These columns:
·         Demonstrate superior performance when simultaneously separating hydrophilic and hydrophobic peptides, as well as neutral and highly basic peptides
·         Perform better at high and low mass load conditions

·         Are the best solution for validation compliance.

 

 

Symmetry300 C₄ columns have the highest average peak capacity compared to over 20 leading brands of HPLC columns. In the example shown, a difficult protein separation, the Symmetry300 C₄ column provided a better separation with better peak shape compared to a leading competitive 300Å column. 

Protein: Symmetry300 C₄ Versus Competitors 

Waters Cartridge Column

Waters cartridge system requires re-usable endfittings, which you order when purchasing a Waters HPLC cartridge column for the first time.  Connect these cartridge columns to your HPLC instrument using Waters standard connectors.

 

Cartridge End-Fittings
2.1 x 50 mm, 2.1 x 100 mm, 2.1 x 150 mm, 2.1 x 250 mm use Part Number 700000117
3.0 x 50 mm, 3.0 x 100 mm, 3.0 x 150 mm, 3.0 x 250 mm use Part Number WAT037525
3.9 x 50 mm, 3.9 x 100 mm, 3.9 x 150 mm, 3.9 x 250 mm use Part Number WAT037525
4.6 x 50 mm, 4.6 x 100 mm, 4.6 x 150 mm, 4.6 x 250 mm use Part Number WAT037525
Waters Sentry Guard Column and Universal Guard Holder
Waters offers a line of Sentry™ Guard columns that contain the same high-performance packing materials as all Waters analytical columns. 
Developed for customers needing increased capacity for biological fluid cleanup and/or temperature control, the Sentry Guard column is available in two holder designs:
·         For use as an integrated part of the Waters steel cartridge column with reusable endfitting
·         For use with any HPLC column.
Both are installed without tools.  
Integrated Sendtry Guard Holder to go with cartridge end-fittings (see above).

3.0 x 20 mm, 3.9 x 20 mm use Part Number WAT046905

Universal Guard Holder (Use on any column)
2.1 x 10 mm use Part Number WAT097958
2.1 x 20 mm use Part Number 186000262
3.0 x 20 mm use Part Number WAT046910
3.9 x 20 mm use Part Number WAT046910
4.6 x 20 mm use Part Number WAT046910