Sialic Acid and Monosaccharide Analyses

Orthogonal Techniques for Quantitation

Evidence for reliable and consistent glycosylation of glycoprotein therapeutics is typically obtained through LC or LC-MS analysis of released N-linked glycans. However, drug discovery and development organizations, as well as regulatory agencies, frequently find value using orthogonal techniques to determine and/or confirm quantitative levels of sialic acids and monosaccharides contained in a therapeutic protein due to potential effect on biological activity and stability of the drug.

Waters offers UPLC, UHPLC, and HPLC-based C18 columns and defined application conditions that can assist scientists with the analysis and quantitation of these important glycan derived components.

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