ProteinWorks

Reproducible and Standardized Protein Quantification with a Simplified Kit-Based Approach

Routinely achieve reproducible, accurate, and sensitive protein quantification with ProteinWorks, Waters new family of protein bioanalysis sample preparation kits. ProteinWorks eXpress Digest Kits simplify and accelerate protein digestion, streamlining, and standardizing the traditionally complex pre-analytic workflow for LC-MS protein quantification via the surrogate peptide approach. The ProteinWorks uElution SPE Kit is designed for post-digestion clean-up, increasing assay sensitivity, and improving system robustness by removing excess digest reagents, phospholipids, and other plasma and serum components.

With ProteinWorks, achieve:

Simplified sample preparation with pre-measured, lot-traceable reagents
Reproducible and accurate results for several monoclonal antibodies
Reduced time to results and cost of analysis

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Demonstrated Inter-Kit and Intra-Kit Reproducibility using ProteinWorks eXpress Direct Digest Kit. CVs of < 6% for 5 different kits and 5 Avastin peptides.

Reproducible

Optimized kit-based approach delivers mean %CVs of < 6% using multiple peptides for 4 representative mAbs in plasma
Streamlined protocol reduces the number of sample preparation steps
Inter-kit and intra-kit precision ensures reproducible results, day to day, lab to lab, and analyst to analyst

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Sensitive

High sensitivity detection limit of 10 ng/ml was achieved for infliximab in rat plasma
Kits are optimized for the digestion of whole plasma or immunopurified samples
uElution SPE improves assay sensitivity through sample concentration and the removal of salts, phospholipids, and residual digestion reagents

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Chromatogram showing 10ng/ml of infliximab in rat plasma, as compared to blank rat plasma. Infliximab is quantified using the unique peptide SINSATHYAESVK

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Standardized & Simplified

Broadly applicable protocol eliminates Method Development for Discovery Studies
Kit-based approach is easily deployable from lab to lab, site to site, and sponsor to CRO
Simple, well-defined protocols and pre-measured lot-traceable reagents facilitate method transfer and provide a well-researched starting point for those new to protein bioanalysis

Flexible

Convenient flexible format easily accommodates 1-96 samples with (12) strips of (8) 1 mL tubes
For optimal results, 3 and 5 step protocols offer the choice to reduce and alkylate, or not
96-well format is easily automatable

For Post-Digestion Sample Clean-Up, use the ProteinWorks uElution SPE Kit

(sold separately or included in ProteinWorks eXpress Digest and eXpress Direct Digest Start-up Kits)

Optional intact murine mAb standard is ideal for workflow training and system health checks

(sold separately or included in ProteinWorks eXpress Digest and eXpress Direct Digest Start-up Kits)

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ProteinWorks eXpress Digest Demonstration Video

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Webinar: Towards Standardization of High Performance Protein Quantification Worksflows

Download the ProteinWorks Application Notebook

Webinar: Improvements in Protein Quantification start with Sample Preparation