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Protein Size-Exclusion Chromatography (SEC)

Size exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii versus absolute molecular weight) in solution with larger sized species eluting before smaller proteins.  The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. As the molecular weight of the proteins of interest increases, SEC columns containing comparatively larger pore size particles are selected.

Why choose BEH SEC column technology:

  • Less ionic interactions with stable Diol-coating for confidence in recovery and reproducibility
  • Unlike silica-based particles, BEH is pH stable >pH 10 for method flexibility
  • Outstanding column lifetime
  • Scalable chemistries for method transfer
  • Pore size options that include 125 Å, 250 Å and 450 Å depending on the protein of interest
 
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