Protein SEC Columns

Size-Exclusion Chromatography

Size exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii) in solution and not by absolute molecular weight with larger sized species eluting before smaller proteins.  The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. SEC columns containing comparatively larger pore size particles are selected as the molecular weight of the proteins of interest increase.

Key Aspects to Consider:

  • Pore size and column dimension is based on the complexity of sample to be separated
  • LC System dispersion used for the analysis, as well as sample throughput
  • Based on these criteria, the most appropriate UPLC, UHPLC, or HPLC offering can be selected
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SEC Optimization SEC Optimization Guide

Helpful considerations for robust UPLC-based SEC protein separations.
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Bioseparations and Analyses Catalog 2019/20 Bioseparations Catalog

HPLC, UHPLC, UPLC, and LC-MS consumables for biomolecule analyses.
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