Protein-Pak LC Columns contain either well-performing silica or methacrylate-based, ion-exchange, size-exclusion, and hydrophobic interaction, and affinity column chemistries for the separation, lab-scale purification, and characterization of proteins and other biomolecules.
The Protein-Pak HPLC and UPLC Column family includes:
Protein-Pak Hi Res Hydrophobic Interaction Chromatography (HIC) Column and HIC Protein StandardProtein-Pak Hi Res HIC Columns contain non-porous, polymethacrylate-based particles (2.5 μm) functionalized with a butyl ligand coating and are well suited for the characterization of proteins and biotherapeutics including monoclonal antibodies (mAb) and antibody drug conjugates (ADC).
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Protein-Pak Hi Res Ion-Exchange (IEX) ColumnsProtein-Pak Hi Res IEX Columns were developed to assist in the characterization of recombinant proteins, monoclonal antibodies, and other biological compounds. The non-porous, high compound binding capacity of these particles yields outstanding resolution of charged species in less time compared to use of many traditional porous IEX offerings. In addition, quality control testing with defined protein standards helps ensure consistent batch-to-batch performance.
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Protein-Pak HR Ion-Exchange Glass ColumnsProtein-Pak HR packing materials are based on rigid, hydrophilic, polymethacrylate particles with large 1000 Å pores. The naturally hydrophilic polymer reduces non-specific adsorption, resulting in quantitative recovery of protein mass and bioactivity. These packings are compatible with buffers in the pH range 2-12, and will withstand exposure to caustic solutions. Protein-Pak HR ion exchangers are available with a:
The Protein-Pak HR 8 µm and 15 µm packing materials are available prepacked in Waters Advanced Purification (AP) glass columns. These columns are compatible with a variety of analytical and preparative LC instrumentation. |
Protein-Pak Size-Exclusion Chromatography (SEC) ColumnsProtein-Pak packings are based on a 10 μm diol-bonded silica and are available in a variety of pore sizes and column configurations. The Protein-Pak SEC Columns:
The degree of resolution is more dependent on the sample mass and volume than the interaction between the sample and the stationary phase. Ideally, there should be no interaction between the stationary phase and the sample molecules. Potential interactions are reduced by adding salts in the 0.1–0.3 M concentration range. |
Affinity Epoxy-Activated ColumnsProtein-Pak affinity epoxy-activated packing consists of 40 µm, 500Å pore size particles that have a hydrophilic bonding layer with a glycidoxypropyl functionality, resulting in a seven atom spacer arm. The epoxy-activated surface can immobilize a wide range of ligands via a covalent linkage with amino, hydroxyl or sulfhydryl groups using simple coupling procedures. For method screening or small scale separation, choose the convenience of prepacked micro columns. Larger scale separations are easily achieved by packing bulk material in our Advanced Purification (AP) glass column. To estimate packed bed volume for a known amount of Protein-Pak affinity epoxy-activated packing: Protein-Pak affinity epoxy-activated used (g) x 2 = packed bed volume (mL). |