IEX separations are most commonly performed using gradients of increasing salt, changing pH, or simultaneous salt increases and pH changes with less charged protein species eluting prior to more highly charged molecules. Based on protein type and separation pH, either an anion or cation exchanger is selected for the separation. In addition, gradient duration, buffer composition and pH, flow rate, as well as separation temperature, all play an important part in obtaining desired protein separations.
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BioResolve SCX mAb ConsumablesCharge variant profiling is one of several techniques performed to assess the efficacy and safety of therapeutic monoclonal antibodies. To address this need, Waters developed BioResolve SCX mAb, 3 μm, corrosion–resistant columns which contain non-porous, polymer-based particles grafted with a rigorously-optimized, multi-component network of negatively charged sulfonic acid ligands. This innovative column technology delivers reproducible, high-resolution, charge-based separations of mAbs and other proteins in LC and LC-MS applications using robust salt or pH gradient methods.
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mAb Charge Variant StandardThis standard is specifically designed to help benchmark charge variant chromatography when using the BioResolve SCX mAb Columns. It contains a unique mix of intact proteins relevant to biopharmaceutical customers: trytophan, conalbumin, and the NIST RM 8671 (NISTmAb). In addition, check out our other product offerings for IEX test standards that were designed for either cation- or anion-exchange chromatography with a different set of three proteins that are used to test the Protein-Pak Column line. |
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Auto•Blend Plus TechnologyAuto•Blend Plus Technology significantly reduces the time you spend in the prep lab by automating the formulation of mobile phases from reservoirs of pure solvents or concentrated stock solutions. It enables you to take full advantage of the ACQUITY UPLC H-Class System's quaternary blending capabilities. Auto•Blend Plus manages the desired pH and ionic strength requirements for the selected mobile phase. Auto•Blend Plus is a standard feature of the ACQUITY UPLC H-Class, ACQUITY UPLC H-Class Bio Systems, and ACQUITY Arc Systems. |
Protein-Pak Hi Res Ion-Exchange (IEX) ColumnsProtein-Pak Hi Res IEX Columns were developed to characterize recombinant proteins, monoclonal antibodies, and other biological compounds. Their non-porous, high compound binding capacity yields outstanding resolution of charged species in less time than traditional porous IEX offerings.
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Protein-Pak HR Ion-Exchange Glass ColumnsProtein-Pak HR Ion-Exchange Glass Columns are packed with rigid, polymethacrylate particles with large 1000 Å pores. The naturally hydrophilic polymer reduces non-specific adsorption, resulting in quantitative recovery of proteins while maintaining bioactivity. The Protein-Pak HR material is stable at pH values from 2-12 and withstands exposure to caustic solutions. Protein-Pak HR series packing materials include:
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BioSuite Ion-Exchange HPLC ColumnsCation and anion-exchange columns (polymethacrylate based) BioSuite Ion-Exchange (IEX) Columns include four separation chemistries -- SP strong and CM weak cation-exchangers, and Q strong and DEAE weak anion-exchangers -- bonded to a pH stable (pH 2–12), methacrylic ester-based polymeric resin. This broad choice of separation chemistries provides chromatographers with the flexibility to develop methods that separate proteins or peptides based on subtle charge differences. Selected BioSuite IEX Columns are also offered with PEEK hardware as well as in 21.5 mm I.D. for lab-scale preparative applications. |
Advanced Purification Glass ColumnsWaters Advanced Purification (AP) Glass Columns are constructed of biocompatible glass and polymeric materials for use with silica, polymer, or soft gel packings. Each column incorporates a distributor to optimize flow and ensure uniform sample distribution onto the packed bed. A replaceable filter protects the packing from large particulate contaminants. Available in a variety of sizes, AP Glass Columns use the same design to ensure predictable methods transfer among them. These columns are compatible with analytical and preparative LC instrumentation. |
The Column: Innovations in IEX for mAb Charge Variant Analysis
Technology Networks: (Part One) Biosimilar Surge Driving Need for Antibody Characterization
Technology Networks: (Part Two) IEX Chromatography Leading the Charge in Charge Variant Analysis
On-Demand Webinar: Advances in IEX for mAb Charge Variant Analysis
HPLC, UHPLC, UPLC, and LC-MS consumables for biomolecule analyses.
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