GlycoWorks RapiFluor-MS N-Glycan Kit

Streamlined Workflow

GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. 

• Easy-to-follow protocol
• Detailed tips and tricks provided for adaptation
• Easily train non-glycan experts

Fast Sample Preparation

The entire process, from glycoprotein to ready-to-analyze samples, can be completed in less than 30 minutes.* This is made possible by fast deglycosylation combined with instant labeling.

• Increase lab efficiency
• Quickly analyze screen samples and provide feedback in a timely manner
• View how it is done with the online demonstration video

Click on the video to the right to watch the latest webinar hosted by GEN.

* Typical for mAb sample, deglycosylation may need to be adapted based on sample.

Unprecedented Sensitivity

RapiFluor-MS is a reagent comprised of an NHS carbamate rapid tagging group, an efficient quinoline fluorophore, and a highly basic tertiary amine for enhancing MS ionization. As an NHS carbamate, unlike alternative rapid tagging reagents, it hydrolyzes over time to generate carbon dioxide and a corresponding amine. This makes RapiFluor-MS labeling a self-quenching reaction. 

Enhanced chemical properties for glycan analysis:

• Rapid tagging
• Efficient fluorescence
• Enhanced ionization efficiency

Phosphoglycan SPE Buffer Improves Recovery

The loss of labeled acidic glycans, especially phosphorylated glycan species, during SPE purification has been considered a challenge to accurately monitor the glycosylation of biotherapeutics. The GlycoWorks Phosphoglycan SPE Elution Buffer, optimized with citrate additive, facilitates the elution and recovery of phosphorylated glycans and achieves maximum yield.

Versatile Formats for 24 to 96 Samples

The GlycoWorks RapiFluor-MS N-Glycan Kits offer two flexible options depending on your sample preparation needs. The 96-sample kits provide enough materials to process 96 samples, 24 at a time. The 24-sample kits provide enough materials to process 24 samples, eight at a time.

• Deglycosylation Module: Rapid PNGase F, customized buffer, RapiGest SF Surfactant, and the Intact mAb Mass Check Standard as a control to benchmark the protocol
• Labeling Module: RapiFluor-MS Reagent and Reagent Solvent
• Clean-up Module: GlycoWorks HILIC µElution Plate* and the GlycoWorks Elution Buffer and Sample Diluent
• Sample Collection Module: Tubes, caps, collection tray, and waste tray needed to handle samples

* A vacuum manifold or positive pressure processor is required for the µElution Plate.

Automation

Waters offers a variety of solutions for workflow automation including the Andrew+ pipetting robot and OneLab, the protocol design and execution software. Additionally, verified scripts for the GlycoWorks RapiFluor-MS method are available for our partner platforms. We provide you with the information needed, no matter the platform, to ensure that you can start achieving your automation results faster.

Andrew+ Pipetting Robot

• Compatible with GlycoWorks standard and automation kits for flexible sample throughput
• Verified protocols available in OneLab
• Compatible with additional connected devices
• Prepare up to 48 samples at a time

Automation partners

• Compatible with GlycoWorks automation kits for high sample throughput
• Starter scripts for Tecan Freedom Evo platform
• Prepare up to 96 samples at a time

Characterization Made Simpler

RapiFluor-MS provides increased fluorescence quantification along with unprecedented mass spectral signal intensity. Low abundance glycans can thus by easily assigned and characterized by serial fluorescence and mass spectrometric detection. Less sample can be used while still obtaining increased sensitivity.

When labeled glycans were analyzed by HILIC chromatography, RapiFluor-MS produced the following results:

• Commercial rapid tagging analog of aminobenzamide (AB); 2x greater fluorescence signal and 1000x higher MS signal
• Conventional reductive amination with 2-AB; 4x greater fluorescence signal and 160x higher MS signal

BioAccord - Reduced Training, Time-Savings, and Consistent Results

The SmartMS™-enabled BioAccord System is an easy-to-use LC-MS platform solution that was purposefully designed for comprehensive analysis of biotherapeutic drug products with built-in analytical workflows for specific analysis such as N-glycan identification and profiling. High quality LC-FLR and LC-MS data can be acquired and transformed into meaningful results for released glycan analysis using an all-encompassing workflow, reducing the cost and time for biotherapeutic development without compromising product quality. For more information, click here.

Assign a Mass to Every Glycan Peak

The ACQUITY QDa Mass Detector routinely detects RapiFluor-MS labeled N-glycans. 

• Equivalent FLR and MS response all within a single run, with the added benefit of mass confirmation
• Enables higher throughput targeted glycan profiling for better informed process development
• Easily integrated with existing GMP compliant-ready UPLC workflows with Empower Software