Size-exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii versus absolute molecular weight) in solution with larger sized species eluting before smaller proteins. The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. As the molecular weight of the proteins of interest increases, SEC columns containing comparatively larger pore size particles are selected.
Why choose BEH SEC column technology?
How to Choose the Right SEC Column for Your AnalysisMaximizing your separation power for challenging analyses such as insulin and monoclonal antibody aggregate analysis is important. Key considerations:
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When Speed is Critical for SECHarnessing the power of UPLC ACQUITY UPLC Technology allows scientists the ability to exceed what is obtainable using UHPLC or traditional HPLC separations by improving data quality while increasing sample throughput and productivity. |
mAb Aggregate, Monomer, and Fragment AnalysesThe BioResolve SEC mAb Guard, Columns, and mAb Size Variant Standard are purposely designed for organizations involved in the research and/or development of monoclonal antibody (mAb) drugs that require accurate quantitation of mAb aggregates (HMWS), monomers, and fragments/clips (LMWS). These offerings provide reliable, high-quality SEC analytical data which addresses internal and regulatory concerns regarding these biotherapeutics. The BioResolve SEC mAb Columns and Guards:
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Adeno-Associated Virus (AAV) AnalysesXBridge BEH SEC 450 Å, 3.5 μm Columns effectively separate and quantitate AAV monomers from their HMW dimers, lower valency multimers, and LMW fragments.
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Insulin Aggregate AnalysisSize-exclusion chromatography (SEC) is the USP and EP standard method for the analysis of covalent HMW insulin in therapeutic preparations. Compared to use of traditional HPLC-based SEC methods, significant improvement in insulin component resolution, while reducing analysis time and mobile-phase consumption, can be obtained using a Waters Protein BEH SEC, 125 A, 1.7 μm Column with Waters UltraPerformance LC (UPLC) instrumentation. |
BEH SEC Protein Performance StandardsBenchmarking, method development, and troubleshooting The BEH SEC Protein Standards are specifically designed to help aid in the benchmarking of each set of columns. Each standard contains carefully chosen proteins unique to that chemistry, which has been worked out meticulously over time. These standards are used by Waters to QC the respective material used to produce the UPLC, UHPLC, and HPLC columns. They are also well suited for benchmarking new columns and for monitoring the SEC column performance during repeated use. |
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Easily Transfer Methods without Compromising on ResolutionFlexibility for UHPLC and HPLC XBridge Protein BEH SEC, 125 Å, 200 Å, and 450 Å Columns containing 2.5 μm (UHPLC) and 3.5 μm (HPLC) particles complement our existing line of UPLC-based SEC offerings for UHPLC or more traditional HPLC-based instrumentation and methods. Easily transfer methods based on laboratory instrumentation, component resolution, or sample throughput needs.
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Important Considerations for SEC Column Selections (Infographic)
A compilation of references to guide you through common challenges and applications associated with peptide and protein SEC analysis.
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Helpful considerations for robust UPLC-based SEC protein separations.
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