• Nota de aplicación

Analysis of Estradiol USP Method Using XBridge C18

Analysis of Estradiol USP Method Using XBridge C18


  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of estradiol USP method using XBridge C18 Columns. 

Introduction

Compounds

Ethylparaben
1. Ethylparaben 
Estradiol
2. Estradiol 
Estrone
3. Estrone 

Experimental

Test Conditions

Column:

XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116

Isocratic Mobile Phase:

water/acetonitrile (45:55)

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Sample Concentration:

20 μg/mL in water/ACN (50:50)

Temperature:

25 ˚C

Detection:

UV @ 205 nm

Instrument:

Alliance 2695 with 2996 PDA

Results and Discussion

1. Ethylparaben 2. Estradiol 3. Estrone.

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WA60164, April 2007

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