• Application Note

Insulin Chain - QC Batch Test for Symmetry300 HPLC Columns

Insulin Chain - QC Batch Test for Symmetry300 HPLC Columns
  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of insulin chain - QC batch test for Symmetry300 HPLC Columns.

Introduction

The compounds used in this study are –

  1. Insulin
  2. Desamino Insulin
  3. Deoxidized Insulin

Experimental

HPLC Method

Column:

Symmetry 300 C4 , 3.9 x 150 mm, 5 μm

Part number:

WAT106154

Mobile phase:

A. 25% of 0.05% TFA in Acetonitrile/75% of 0.05% TFA in Water

B. 32% of 0.05% TFA in Acetonitrile/68% of 0.05% TFA in Water (Linear Gradient from 100% A to 100% B in 14 Min.)

Flow rate:

1.0 mL/min

Injection:

4 μg, 10 μL

Detection:

UV @ 220 nm

Results and Discussion

Featured Products

WA31763.91, June 2003

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