Gradient Separation of Amino Acids on ACQUITY UPLC BEH HILIC

Column:

ACQUITY UPLC BEH HILIC, 2.1 x 50 mm, 1.7 µm

Part Number:

186003460

Mobile Phase A:

10 mM NH₄COOH, 0.2% HCOOH in 50:50 ACN: H₂O

Mobile Phase B:

10 mM NH₄COOH, 0.2% HCOOH in 90:10 ACN: H₂O

Flow Rate:

0.529 mL/min

Injection Volume:

5.0 µL

Sample Concentration:

5 µg/mL

Sample Diluent:

73:25:2 ACN:MeOH:H₂O with 0.2% HCOOH and 5 µM HCl

Temperature:

30 C

Instrument:

Waters ACQUITY UPLC with SQ Mass Detector

0.0

0.1

99.9

1.65

0.1

99.9

4.49

99.9

0.1

4.54

0.1

99.9

5.05

0.1

99.9

Ionization Mode:

ES⁺

Capillary:

3.0 kV

Cone:

20 V

Extractor:

3 V

RF Lens:

0.3 V

Source Temperature:

150 °C

Desolvation Temperature:

350 °C

Cone Gas Flow:

50 L/Hr

Desolvation Gas Flow:

700 L/Hr

SIR:

136.1 m/z Homocysteine

150.2 m/z Methionine