• Application Note

Gradient Chemical Stability Study of ACQUITY UPLC BEH HILIC

Gradient Chemical Stability Study of ACQUITY UPLC BEH HILIC
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the gradient chemical stability study on ACQUITY UPLC BEH HILIC.

Introduction

The compounds used in this study are:

  1. Uracil
  2. 5-Fluorocytosine
  3. Cytosine

Experimental

Test Conditions

Columns:

ACQUITY UPLC BEH HILIC, 2.1 x 50 mm, 1.7 μm

Part Number:

186003460

Mobile Phase A:

95:5 acetonitrile:water with 10 mM NH4+CH3COO- pH 5.5

Mobile Phase B:

50:50 acetonitrile:water with 10 mM NH4+CH3COO- pH 5.5

Flow Rate:

0.5 mL/min

Injection Volume:

2.0 μL (full loop)

Weak Needle Wash:

95:5 acetonitrile:water

Sample Diluent:

75:25 acetonitrile:methanol

Temperature:

30 °C

Detection:

UV @ 254 nm

Sampling Rate:

40 pts/sec

Time Constant:

0.05

Instrument:

Waters ACQUITY UPLC with TUV detector

Gradient

Time (min)

Profile

Curve

%A

%B

0.0

99

1

6

2.0

1

99

6

2.1

99

1

6

2.5

99

1

6

Results and Discussion

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WA60133, August 2009

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