• Application Note

ACQUITY UPLC Analysis of Histidine Dipeptides

ACQUITY UPLC Analysis of Histidine Dipeptides
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application highlights the analysis of Histidine Dipeptides on ACQUITY UPLC BEH Amide Columns.

Introduction

The compounds used in this study are:

  1. Creatinine (1 μg/mL)
  2. Creatine (5 μg/mL)
  3. Anserine (5 μg/mL)
  4. Canosine (5 μg/mL)

Experimental

Chromatographic Conditions

Column:

ACQUITY UPLC BEH HILIC, 2.1 x 50 mm, 1.7 μm

Part Number:

186004800

Mobile Phase A:

50/50 MeCN/H2O with 10 mM CH3COONH4 and 0.04 % NH4OH, pH 9.0

Mobile Phase B:

95/5 MeCN/H2O with 10 mM CH3COONH4 and 0.04 % NH4OH, pH 9.0

Flow Rate:

0.5 mL/min

Injection Volume:

5 μL

Sample Diluent:

75/25 MeCN/MeOH

Column Temperature:

30 °C

Weak Needle Wash:

95/5 MeCN/H2O

Instrument:

Waters ACQUITY UPLC with ACQUITY SQD

Gradient

Gradient

Results and Discussion

Featured Products

WA60103, July 2009

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