Clozapine on Oasis MCX

Oasis MCX 10-mg 96-Well Plate

Condition:

500 μL MeOH

Equilibrate:

500 μL H₂O

Load:

1 mL sample (500 μL human plasma diluted 1:1 with 4% H₃PO₄ in H₂O)

Wash 1:

500 μL 2% HCOOH in H₂O

Wash 2:

500 μL MeOH

Elute:

300 μL (150 μL x 2) 5% NH₄OH in MeOH Dry and reconstitute w/100 μL 0.1% FA in H₂O

Inject:

50 μL

Column:

SunFire C₁₈ 2.1 x 50 mm, 3.5 μm

Mobile Phase A:

0.1% FA in H₂O

Mobile Phase B:

0.1% FA in MeOH

Flow Rate:

0.4 mL /min

Injection Volume:

50 μL

Instrument:

2777 Sample Manager and 1525μ Binary HPLC Pump

ESI+

Source Temp.:

150 °C

Desolvation Temp.:

350 °C

Cone Gas Flow:

50 L /Hr

Desolvation Gas Flow:

600 L /Hr

Collision Cell:

2.2e^-3 bar (Ar gas)