• Application Note

Steroids

Steroids
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of steroids using Sunfire C18 and C8 Columns.

Introduction

The compounds analysed in this study using Sunfire C18 Columns are -

The compounds analysed in this study using Sunfire C8 Columns are -

Experimental

Conditions

Column:

SunFire C18 4.6 x 20 mm IS, 3.5 μm

Part number:

186002549

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 220 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Gradient.

Conditions

Column:

SunFire C8 4.6 x 20 mm IS, 3.5 μm

Part number:

186002699

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 220 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Gradient.

Results and Discussion

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WA41900, March 2005

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