• Application Note

Protriptyline in Rat Plasma by Mixed-Mode Weak Cation Exchange and LC-MS/MS

Protriptyline in Rat Plasma by Mixed-Mode Weak Cation Exchange and LC-MS/MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of protriptyline in rat plasma by mixed-mode weak cation exchange and LC-MS/MS.

Introduction

The compound analyzed in this study is protriptyline.

Experimental

LC Conditions

Column:

XTerra MS C18 2.1 x 20 mm IS, 3.5 μm

Part number:

186001923

Mobile phase A:

10 mM NH4HCO3, pH 10

Mobile phase B:

MeOH with 10 mM NH4HCO3, pH 10

Flow rate:

0.4 mL/min

Injection volume:

10 μL

Column temperature:

Ambient

Instrument:

Waters 2777 Sample Manager and Waters 1525μ Binary HPLC Pump

Gradient

Gradient.

MS Conditions

Waters Micromass Quattro Ultima

ESI+

Source temp.:

150 °C

Desolvation temp.:

350 °C

Cone gas flow:

50 L/Hr

Desolvation gas flow:

550 L/Hr

Collision cell:

2.2e-3 bar (Argon gas)

Cone voltage:

60 volts

CID:

25eV

MRM transition:

m/z 264.0 → 191.1

Results and Discussion

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WA31764.196, June 2003

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