• Application Note

Fluoroquinolone Antibiotics in Beef Kidney by LC-MS/MS

Fluoroquinolone Antibiotics in Beef Kidney by LC-MS/MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights about the analysis of antibiotic in beef kidney by LC-MS/MS.

Introduction

The compounds analyzed in this study are:

  1. Flumequin  
  2. Enoxacin  
  3. Norfloxacin  
  4. Sarafloxacin 
  5. Ofloxacin  
  6. Enrofloxacin  
  7. Danofloxacin  
  8. Lomefloxacin  
  9. Ciprofloxacin

Experimental

LC Conditions

Column:

Atlantis d C18, 4.6 x 150 mm, 5 μm

Part number:

1860001344

Mobile phase A:

0.2% NFPA* in water

Mobile phase B:

Methanol

Gradient:

Linear, 40% B to 80% B in 10 minutes

Flow rate:

0.8 mL/min

Injection volume:

50 μL

Temperature:

30 °C

Instrument:

Alliance 2695 Separations Module

*NFPA- nonafluoropentanoic acid (NFPA) - C4F9COOH

MS Conditions

Instrument:

Quattro

Ion source:

APCI+

Mode:

Multiple Reaction Monitoring

Corona:

0.8 V

Source temp.:

150 °C

Desolvation temp.:

625 °C

Cone gas (N2):

175 L/Hr

Desolvation gas (N2):

250 L/Hr

Collision gas:

Argon

Results and Discussion

Featured Products

WA31764.85, June 2003

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