This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of cephalexin.
The compounds analyzed in this sudy are -
|
Compounds |
USP Tailing Factors |
|---|---|
|
1-hydroxybenzotriazole (IS) |
1.4 |
|
Cephalexin |
1.2 |
|
Column: |
Symmetry C18, 3.0 x 150 mm, 5 μm |
|
Part number: |
WAT054200 |
|
Mobile phase: |
1.25% glacial acetic acid in water/methanol 88:12 |
|
Flow rate: |
0.6 mL/min |
|
Injection volume: |
400 μL of cephalexin (1 mg/mL) and 200 μL of 1-hydroxybenzotriazole (Internal standard, 1 mg/mL) |
|
Detection: |
UV @ 254 nm |
WA31763.51, June 2003