SKU: 186010778
dsDNA 50 to 1350 Ladder

dsDNA 50 to 1350 Ladder | 186010778


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Product Description

The dsDNA 50 to 1350 Ladder is a lyophilized mixture of a proprietary plasmid digest that is comprised of 17 double stranded DNA species, including fragments ranging from 50 to 1,350 base pairs (bp) long. By their higher abundances, the 1350, 500 and 200 bp bands can be used as reference point. The dsDNA 50 to 1350 Ladder is to be used as a performance check and potential system suitability standard for LC-UV analyses, as performed with SEC and IEX separations.

Specifications

  • Separation Mode

    GPC/GFC/SEC

  • Detection Method

    UV

  • Formulation

    Solid

  • Storage Condition

    -20C +/- 10C (Freezer)

  • System Type

    LC

  • Instrument

    UPLC, UHPLC, HPLC

  • Shelf Life

    24 Months

  • Shelf life upon reconstitution

    1 Week

  • Shipping Condition

    Ambient

  • Tolarence

    +/- 20%

  • Type

    Resolution Check

  • UNSPSC

    41116107

  • Application

    Oligonucleotide, Cell & Gene Therapy

  • Brand

    Waters

  • Product Type

    Standards

  • Units per Package

    1 pk

Product Support

Documents

Documents



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dsDNA 50 to 1350 Ladder, 1/pk

The dsDNA 50 to 1350 Ladder is a lyophilized mix of 17 proprietary double-stranded DNA fragments, spanning a size range from 50 to 1350 base pairs (bp). Featuring high-abundance bands at 1350, 500, and 200 bp, it serves as an effective reference tool for LC-UV analysis in both SEC and IEX workflows. These distinct bands aid in reliable size calibration and system performance validation across analytical assays.

This ladder is intended for use as a resolution and system suitability check for oligonucleotide, gene therapy, and cell therapy applications. With a solid formulation and long shelf life of 24 months (1 week post-reconstitution), it is designed to perform under ambient shipping and frozen storage conditions (-20°C). It is compatible with LC systems, including UPLC, UHPLC, and HPLC instruments, and integrates seamlessly into GMP-regulated workflows thanks to Waters' ISO-certified manufacturing standards.

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FAQs

What is the dsDNA 50 to 1350 Ladder used for?
This ladder is used as a resolution and system suitability standard for LC-UV analysis, particularly with SEC and IEX separations.

What sizes of DNA fragments does the ladder contain?
It includes 17 DNA fragments ranging from 50 to 1350 base pairs, with prominent bands at 1350 bp, 500 bp, and 200 bp for easier visualization and reference.

How should the product be stored?
Store at -20°C ± 10°C in a freezer. After reconstitution, the ladder has a shelf life of 1 week.

What types of instruments are compatible with this ladder?
It can be used with a variety of LC platforms including UPLC, UHPLC, and HPLC systems.

Is this product suitable for regulated environments?
Yes, it's produced in an ISO 9000-registered facility following FDA cGMP guidelines, making it suitable for regulated bioanalytical environments.