How Do I Perform Filtration?

Draw approximately 1 mL of air into the syringe before filling the syringe with a sample. This will allow the air to follow the sample out of the syringe. This “air purge” minimizes fluid retention within the filter device. Next, fill the syringe with the solution to be filtered. Holding the filter device in one hand, secure (without excessive force) the filled syringe to the filter device with a twisting motion. Apply gentle pressure to begin filtration.

What Is Sensitivity In Chromatography?

Sensitivity is the signal output per unit concentration, or unit mass of a substance in the mobile phase entering the detector, e.g., the slope of a linear calibration curve. Sensitivity is also the ratio of peak height to the analyte concentration in the peak for concentration-sensitive detectors. It is the ratio of peak height to unit mass in mass-flow-sensitive detectors. For sensitivity to be a unique performance characteristic, it must depend only on the chemical measurement process, not on scale factors. The ability to detect (that is, qualify) or measure (that is, quantify) an analyte is dependent on many instrumental and chemical factors. In the ideal scenario, well-resolved peaks elute from high-efficiency columns (narrow peak width with good symmetry for maximum peak height). This is in addition to good detector sensitivity and specificity. To achieve maximum sensitivity, both the separation system interference and electronic component noise should also be minimized.