Protein-Pak SP 5PW Column, 10 µm, 7.5 mm x 75 mm, 1/pk

Designed for legacy methods that demand precision and reliability, the Protein-Pak SP 5PW Column offers robust performance for ion-exchange chromatography. This 10 µm spherical polymer-based strong cation-exchange column is engineered for the separation and analysis of proteins in high-performance liquid chromatography (HPLC) workflows. Its large 1000 Å pore size and broad pH operating range of 2 to 12 make it ideal for handling a wide range of biomolecules, from 1,000 to 500,000 molecular weight.

With a 7.5 mm inner diameter and 75 mm length, this column provides excellent scalability and resolution in preparative applications, especially when working with older or validated protocols that require consistent retention times and binding capacity. Its Parker-style endfitting ensures compatibility with established systems, and the column's polymer matrix supports high chemical stability across challenging mobile phases.

The Protein-Pak SP 5PW Column is part of the trusted Protein-Pak line, supporting protein purification and characterization with superior performance and reproducibility. Whether you're running ion-exchange methods in pharmaceutical R&D or legacy QC protocols, this column is designed to maintain the integrity of your process.

Looking to stock up on additional HPLC tools or compatible hardware? You can shop for lab equipment for all lab needs.

Frequently Asked Questions

What applications is the Protein-Pak SP 5PW Column best suited for?
This column is ideal for strong cation-exchange separation of proteins, particularly in legacy HPLC workflows, preparative protein purification, and methods validated under older regulatory standards.

What is the particle composition and why does it matter?
The column uses a polymer-based spherical particle, which offers chemical robustness and durability over a broad pH range. This makes it particularly suited for harsh mobile phases and long-lasting performance.

Can this column be used in modern HPLC systems?
Yes, the column's Parker-style endfitting and 7.5 mm format make it compatible with most standard HPLC setups. It integrates well into both older and modern systems without hardware modification.