Protein-Pak 125 Column, 10 µm, 7.8 mm x 300 mm, 1/pk

With packings based on a 10 µm diol-bonded silica, Protein-Pak 125 Columns are made to resolve proteins that differ in molecular weight by a factor of two, as well as distinguishing proteins that differ by as little as 15% in molecular weight. This allows for the separation of biopolymers, including proteins and enzymes, in HPLC applications. The degree of resolution will be dependent on the sample mass and the volume more than the interaction between sample and stationary phase.

Protein-Pak 125 Columns are suited to the following weight ranges: Native Globular 2,000 – 80,000 and Random Coil 1,000 – 30,000. Columns are shipped in 100% methanol and contain one column per order. The inner diameter of these columns is 7.8 mm with a length of 300 mm.

In ideal scenarios, there will be no interaction between the stationary phase and the sample molecules. Because secondary interactions are most often ionic, they can usually be reduced by increasing the ionic strength of the mobile phase. Typical salt concentrations range from 0.2 – 0.5 M NaCl. It could also be useful in some cases to consider adding 10-20% methanol to eliminate hydrophobic and other hydrogen bonding interactions.

For decades, Waters has offered continuous improvements to SEC, with refined instrumentation, packing materials, and technologies. This allows for innovations that expand size-exclusion techniques beyond the original polymer analysis. Like all Waters lab equipment, Protein-Pak 125 Columns are thoroughly tested in a quality-controlled laboratory to ensure adherence to stringent specifications. These products work well in conjunction with the Protein-Pak SP 5PW Glass, 8 mm x 75 mm, 1/pk, among other Waters’ products.