Protein-Pak SEC Column, 300Å, 10 µm, 7.5 mm X 300 mm, 10K - 500K, 1/pk

Add the Waters Protein-Pak SEC Column to your lab equipment collections as it is the column of choice for use with larger biological active molecules in the molecular weight range of Native Globular 10,000-400,000 and Random Coil 2000-150,000. The Protein-Pak SEC Column is shipped in 0.05% sodium azide in a water solution.

Resolve proteins that differ in molecular weight easily by a factor of two while distinguishing proteins that differ by as little as 15% in molecular weight. The degree of resolution depends more on the sample mass and volume than the interaction between the sample and the stationary phase. As secondary interactions are most often ionic, by increasing the ionic strength of the mobile phase, they can be reduced, for instance, by using salt concentrations in the 0.2-0.5M range (NaCl). In some cases, you can also use 10-20% methanol in order to eliminate hydrophobic and other hydrogen-bonding interactions.

To install the steel Protein-Pak SEC Column, start by removing the end plugs from your column with a 5/18” wrench and save them for storage when the column is removed from the system. The column outlet is indicated by an arrow on the label, which indicates the direction solvent should flow. Tighten the fittings 1/4-to-1/2 turn, but do not overtighten as this will damage the fitting seat. A properly prepared and assembled compression fitting in good conditions is all that is required.

The lab equipment is based on 10 µm diol-bonded gel filtration materials and is also available in a range of pore sizes and column configurations that you can explore and shop for using our online store. You may also wish to explore similar products and those that can be used alongside this Protein-Pak SEC Column, such as the MassPrep Protein Standard Mix.