Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
15 µm |
Pore Size |
100 Å |
Format |
Radial-Pak Guard |
Surface Area |
300 |
System |
HPLC |
USP Classification |
L26 |
Inner Diameter |
25 mm |
Length |
10 mm |
Carbon Load |
7 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
Delta-Pak |
Product Type |
Guards |
Units per Package |
2 pk |
Delta-Pak C4 Radial-Pak Guard, 100Å, 15 µm, 25 mm x 10 mm, 2/pk
The Delta-Pak C4 Radial-Pak Guard delivers reliable protection for reversed-phase separations involving proteins, peptides, and other hydrophobic compounds. Built on 15 µm endcapped spherical silica particles with a 100 Å pore size, this guard column helps preserve the performance of your analytical system, especially during high-throughput workflows or when handling complex samples. The bonded C4 chemistry offers stable retention with low silanol activity, while the 25 mm inner diameter and 10 mm length ensure minimal disruption to your method's efficiency or resolution.
With an operating pressure tolerance of up to 6000 psi, this guard is well-suited for rugged protein separations and routine assays alike. The Radial-Pak format streamlines cartridge changes, reducing system downtime and maximizing operational convenience. Whether you're screening samples or scaling up from analytical to preparative purification, this guard column helps maintain column integrity and consistency from run to run.
Pair it with trusted accessories like the LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vials for sample handling, or explore more tools in our full lab equipment catalog to keep your workflow running strong.
What does the Delta-Pak C4 Guard Column protect against?
This guard column defends your main analytical column from particulates, contaminants, and buildup that can degrade performance over time. It extends column life while supporting reproducible results.
When should I replace my Radial-Pak Guard?
Replace it once you notice increased system backpressure or a drop in resolution. Guard columns are designed to be replaced more frequently than primary columns to maintain optimal performance.
Is this guard compatible with all HPLC systems?
Yes, its standard Radial-Pak format and dimensions integrate easily with most HPLC setups, especially those already using Delta-Pak or similar reversed-phase workflows.
What types of applications benefit from this C4 chemistry?
The C4 phase is ideal for hydrophobic molecules like peptides and proteins. It's commonly used in biopharmaceutical testing, peptide mapping, and natural product analysis.