On Average, How Long Does It Take To Change A Column?

Column changes are a practical approach for method development in HPLC, allowing for flexibility in switching columns as required. To attach a new column, it is recommended to introduce a slow flow of solvent from the injector outlet line to fill the inlet fitting of the column. This ensures that no air is left in the column entrance. For re-equilibration, it is advisable to flush the column with a minimum of six column volumes of the new solvent before injecting a sample. Typically, the first injection may be disregarded, as subsequent injections (such as the second, third, and onwards) tend to exhibit reproducibility. By following this procedure, you can effectively perform column changes and ensure proper column performance for your HPLC analysis.