Chemistry |
Silica |
Separation Mode |
Normal-Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Bonding Technology |
Silica |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Parker-style |
Pore Size |
80 Å |
Format |
Column |
Surface Area |
220 |
System |
HPLC |
Particle Technology |
Silica |
USP Classification |
L3 |
Inner Diameter |
4 mm |
Length |
250 mm |
UNSPSC |
41115709 |
Brand |
Spherisorb |
Product Type |
Columns |
Units per Package |
1 pk |
Spherisorb Silica Silica Column, 80Å, 5 µm, 4 mm X 250 mm, 1/pk
Opt for the Waters Spherisorb Silica Silica Column to benefit from better efficiency, peak shape, reproducibility, and lifetime. Spherisorb silica chemistry is a general purpose unbonded silica substrate designed for normal phase applications. It addresses common process parameters such as slurry solvent choice, packing pressure, time, temperature, flow rate, and slurry volume to deliver the most reliable lab equipment.
The Spherisorb Silica packing materials are produced in a cGMP, ISO 9001-certified facility using ultra-pure reagents and created expressly for use with the ACQUITY UPLC System. To ensure repeatable performance, each batch of Spherisorb Silica Column packing material is chromatographically tested with acidic, basic, and neutral analytes, and the results are held to limited specification ranges. On the eCordTM Intelligent Chip, a Performance Chromatogram and Certificate of Batch Analysis are supplied after each column has undergone an individual test. On the test chromatogram, the following information is included: gel batch number, column serial number, USP plate count, USP tailing factor, and chromatographic conditions. These data should be stored for future use.
The Spherisorb lab equipment is available in a wide variety of particle sizes and chemistries, so you have flexibility for method development. You can browse the entire range as well as shop for lab equipment using our website.
You may also want to purchase the Spherisorb Silica Silica Guard Cartridge, 80Å, 5 µm, 4.6 mm X 10 mm, 3/pk. It is made using the exact packing materials that are used in the column and works by removing any chemical or particulate matter from the mobile stream. This results in more accurate and reliable analysis while also enhancing the lifetime of the column.
What Is The pH Range Offered By The Spherisorb Silica Silica Column?
The Spherisorb Silica Silica Column listed here is able to function well across the 2-8 pH range. In addition to this, it is important to remember that the column’s lifetime can be impacted by the type of operating temperature, as well as the type and concentration of the buffers that are used. For instance, using a phosphate buffer at 8 pH combined with high temperatures can result in a shorter column lifetime.
What Pressure Can The Spherisorb Silica Silica Column Sustain?
The Spherisorb Silica Silica Column listed here is able to tolerate a maximum of 6000 psi (415 Bar) pressure.