Chemistry |
Anion-Exchange |
Separation Mode |
Ion-Exchange |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Bonding Technology |
SAX |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Parker-style |
Pore Size |
80 Å |
Format |
Column |
Surface Area |
220 |
System |
HPLC |
USP Classification |
L14 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
4 % |
UNSPSC |
41115709 |
Brand |
Spherisorb |
Product Type |
Columns |
Units per Package |
1 pk |
Spherisorb SAX Column, 80Å, 5 µm, 4.6 mm X 150 mm, 1/pk
Waters Spherisorb columns are one of the most commonly referred to HPLC columns in the scientific literature. Over 2000 analytical abstracts have been published on Waters Spherisorb columns, providing you with a diverse range of validated techniques and applications to aid in method development. These columns come with Parker-style tube/column end fittings (or cartridge end fittings). A silica-based quaternary ammonium-bound sorbent is used in the Spherisorb SAX columns. The column, designated USP L14, is utilized for anion-exchange separations.
The Spherisorb SAX Column listed here can support particle sizes up to 5 µm, maximum pressure of 6000 psi (415 Bar), and a pH range of 2 to 8, but it is always recommended to keep operating conditions within the given constraints because exposure to extreme pressures, pH, or even temperatures will result in shorter column lifetimes and significantly impact performance. Use the Spherisorb SAX Column to dependably meet liquid chromatography quality standards.
Spherisorb SAX Columns have been meticulously engineered to be general-purpose, silica-based, long-lasting, and extremely efficient chromatographic columns. These columns are made at Waters chromatography chemistry plants that are specialized and tightly supervised to maintain the highest and toughest industry conditions to meet cGMP and ISO 9002. As a result, you can count on the Spherisorb SAX Columns to provide the most important performance characteristics, such as peak symmetry, column longevity, column efficiency, and column-to-column repeatability.
With the correct lab equipment, you can boost your production and efficiency. Check out our shop for lab equipment to review the various lab equipment offerings from Waters, such as the Spherisorb SAX Column, or to locate additional goods that are compatible with it; you may browse around our website, refer to our catalog, and ensure your lab needs are addressed as needed.
You should also look at the LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; The manufacturing methods and handling procedures for LCMS Certified vials are strictly supervised. They are mass spectrometer tested and come with a certificate of analysis displaying the reference and vial scan for the production batch.
What Effect Does Temperature Have on Liquid Chromatography?
The process of chromatography involves a sequence of dynamic interactions where analytes can either dissolve in the mobile phase or adhere to the stationary phase within the column. The transfer of analytes between the mobile and stationary phases becomes more rapid with increasing temperature. Consequently, elevated temperatures expedite the analysis procedure.