Chemistry |
Amino (NH2) |
Separation Mode |
Normal-Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Parker-style |
Pore Size |
80 Å |
Format |
Column |
Surface Area |
220 |
System |
HPLC |
USP Classification |
L8 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
Carbon Load |
2 % |
UNSPSC |
41115709 |
Brand |
Spherisorb |
Product Type |
Columns |
Units per Package |
1 pk |
Spherisorb Amino (NH2) Column, 80Å, 5 µm, 4.6 mm X 250 mm, 1/pk
The Spherisorb Amino (NH2) Column is the perfect lab equipment for most normal phase chromatography applications. It is also well suited for the stationary phase of HILIC (Hydrophilic Interaction Chromatography) for positive phase analysis of polar compounds. In addition to this, you can rely on the Spherisorb Amino (NH2) Column for analysis of weak anion exchange, as well as aqueous reversed-phase liquid phase analysis.
The Spherisorb Amino (NH2) Column has a wide range of applications and can be used for the analysis of low molecular weight sugars such as glucose, fructose, xylose, and lactose. It is a suitable replacement for most of the amino liquid chromatography columns on the market.
The Spherisorb Amino (NH2) Column offers chemical stability across a range of pH points, with minimum pH being 2 and the maximum being 8. It also offers a maximum operating pressure of 6000 psi (415 Bar).
The Spherisorb Amino (NH2) Column comes with industry-standard end fittings. The threaded female fitting requires a Parker-style compression screw and ferrule to connect the HPLC tubing to the analytical column. Therefore, if your instrument is set up for Waters’ columns, replace your ferrule on the connector tubing and set it for the Parker standard.
You may also be interested in Spherisorb Amino (NH2) Guard Cartridge, 80Å, 5 µm, 4.6 mm X 30 mm, 3/pk. The guard cartridge is specially designed to work with the Spherisorb Amino (NH2) Column and ensures that your analytical column lasts a long time by removing any chemical or particulate contaminants from the mobile phase.
To find other lab equipment that may be related to the listed product or otherwise suitable for your lab, browse through our catalog that contains the complete available product range as well as the latest updates on research regarding lab equipment. It also carries contact details and enables you to shop for lab equipment.
What Temperature Is Recommended For Spherisorb Amino (NH2) Column?
The recommended operating temperature range for Spherisorb Amino (NH2) Column is between 20 - 45°C. Using this temperature range will ensure enhanced selectivity, lower solvent viscosity, and increased mass transfer rates. However, any temperature above ambient may have a negative effect on lifetime which will vary depending on the pH and buffer conditions used.